How to detect the spill-over of glutamate ? Detection of extra-synaptic receptor using MK801 (Westbrook, Neuron02) Glutamate uptake controls spill-over. Low stim decays faster than high stim (Scanziani, NN02) Neuronal Glutamate Transporter…

Transfect Hela Cell with Sensors. Measure slices Very funny result. Some delayed activity was observed. Maybe cAMP dependent pathway ?