Paper

Scott,D.B., and Ehlers, M.D. et al. (2004) "Endocytosis and Degradative sorting of NMDA receptors by conserved membrane-proximal signals" J. Neurosci. 24:7096-7109
[Notes] Nice story about mechanisms of regulation of NMDA receptors. The paper showed membrane-proximal site in NR1 C-tail is sufficient for endocytosis of NR1/NR2 complex. Similarly, membrane-proximal region of NR2 C-tail enhances the internalization of NR1/NR2 complex in additive manner. Distal region of NR2 C-tail is required for recycling of internalized receptors.
To monitor internalization, they labeled surface YFP-tagged receptors with anti-GFP alive and incubated cells at 37 deg, allowing cells to undergo endocytosis. To monitor recycling, the remaining surface antibody is stripped, and unstripped antibody is blocked by unconjugated secondary antibody. Cells are incubated further to allow recycling. Surface antibody are then labled with fluorecence secondary antibody.